TABLE 1.

Total recovery of substance in the system after nebulization and deposition on the apical side of permeable supports.

	20 μl	100 μl	200 μl
Nebulizer	20.5 ± 12.2	34.2 ± 2.83	27.2 ± 3.64
Device	45.8 ± 9.37	55.4 ± 3.32	63.0 ± 8.77
Transwell	5.52 ± 0.84	3.31 ± 0.54	3.43 ± 0.23
Total recovery	79.7 ± 9.02	92.9 ± 2.02	93.5 ± 8.51

20, 100, and 200 μl of sodium fluorescein solution are nebulized at 25 μg/ml as described in the section “Materials and Methods.” The relative abundance of deposited substance in each part of the system is displayed for each nebulized volume. “Total recovery” is the sum of the relative abundance of each invested volume. Error represents standard deviation. N = 9 of three experiments; 100 μl, N = 6 of three experiments.