Table 2.
The oligonucleotides sequences and thermal-cycling conditions of PCR assay.
Genes | Oligonucleotides sequences | Amplified product (bp) | PCR conditions (35 cycles) | References | ||
---|---|---|---|---|---|---|
Denaturation | Annealing | Extension | ||||
atpD |
GTATCATGAACGTTCTGGGTAC TGAAGTGATACGCTCTTGCAG |
595 |
94 °C 30 s |
58 °C 40 s |
72 °C 45 s |
25 |
ureC |
GTTATTCGTGATGGTATGGG ATAAAGGTGGTTACGCCAGA |
317 |
94 °C 30 s |
56 °C 40 s |
72 °C 40 s |
30 |
rsbA | TTGAAGGACGCGATCAGACC ACTCTGCTGTCCTGTGGGTA | 467 |
94 °C 30 s |
58 °C 40 s |
72 °C 45 s |
30 |
zabA |
ACCGCAGGAAAACATATAGCCC GCGACTATCTTCCGCATAATCA |
540 |
94 °C 30 s |
59 °C 40 s |
72 °C 45 s |
30 |
tetA |
GGTTCACTCGAACGACGTCA CTGTCCGACAAGTTGCATGA |
576 |
94 °C 30 s |
50 °C 40 s |
72 °C 45 s |
31 |
sul1 | CGGCGTGGGCTACCTGAACG GCCGATCGCGTGAAGTTCCG | 433 |
94 °C 30 s |
54 °C 40 s |
72 °C 45 s |
32 |
blaKPC |
ATGTCACTGTATCGCCGTCT TTACTGCCCGTTGACGCCC |
892 |
94 °C 1 min |
55 °C 1 min |
72 °C 1 min |
33 |
blaNDM-1 |
GGCGGAATGGCTCATCACGA CGCAACACAGCCTGACTTTC |
287 |
94 °C 30 s |
55 °C 30 s |
72 °C 30 s |
33 |
blaCTX-M |
ATG TGC AGY ACC AGT AAR GTK ATG GC TGG GTR AAR TAR GTS ACC AGA AYC AGC GG |
593 |
94 °C 30 s |
54 °C 40 s |
72 °C 45 s |
34 |
blaOXA-1 |
ATATCTCTACTGTTGCATCTCC AAACCCTTCAAACCATCC |
619 |
94 °C 30 s |
54 °C 40 s |
72 °C 45 s |
35 |
blaTEM |
ATCAGCAATAAACCAGC CCCCGAAGAACGTTTTC |
516 |
94 °C 30 s |
54 °C 40 s |
72 °C 45 s |
35 |