Skip to main content
. 2021 May 4;11:9476. doi: 10.1038/s41598-021-88861-w

Table 2.

The oligonucleotides sequences and thermal-cycling conditions of PCR assay.

Genes Oligonucleotides sequences Amplified product (bp) PCR conditions (35 cycles) References
Denaturation Annealing Extension
atpD

GTATCATGAACGTTCTGGGTAC

TGAAGTGATACGCTCTTGCAG

595

94 °C

30 s

58 °C

40 s

72 °C

45 s

25
ureC

GTTATTCGTGATGGTATGGG

ATAAAGGTGGTTACGCCAGA

317

94 °C

30 s

56 °C

40 s

72 °C

40 s

30
rsbA TTGAAGGACGCGATCAGACC ACTCTGCTGTCCTGTGGGTA 467

94 °C

30 s

58 °C

40 s

72 °C

45 s

30
zabA

ACCGCAGGAAAACATATAGCCC

GCGACTATCTTCCGCATAATCA

540

94 °C

30 s

59 °C

40 s

72 °C

45 s

30
tetA

GGTTCACTCGAACGACGTCA

CTGTCCGACAAGTTGCATGA

576

94 °C

30 s

50 °C

40 s

72 °C

45 s

31
sul1 CGGCGTGGGCTACCTGAACG GCCGATCGCGTGAAGTTCCG 433

94 °C

30 s

54 °C

40 s

72 °C

45 s

32
blaKPC

ATGTCACTGTATCGCCGTCT

TTACTGCCCGTTGACGCCC

892

94 °C

1 min

55 °C

1 min

72 °C

1 min

33
blaNDM-1

GGCGGAATGGCTCATCACGA

CGCAACACAGCCTGACTTTC

287

94 °C

30 s

55 °C

30 s

72 °C

30 s

33
blaCTX-M

ATG TGC AGY ACC AGT AAR GTK ATG GC

TGG GTR AAR TAR GTS ACC AGA AYC AGC GG

593

94 °C

30 s

54 °C

40 s

72 °C

45 s

34
blaOXA-1

ATATCTCTACTGTTGCATCTCC

AAACCCTTCAAACCATCC

619

94 °C

30 s

54 °C

40 s

72 °C

45 s

35
blaTEM

ATCAGCAATAAACCAGC

CCCCGAAGAACGTTTTC

516

94 °C

30 s

54 °C

40 s

72 °C

45 s

35