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. 2021 May 4;12:2508. doi: 10.1038/s41467-021-22815-8

Fig. 6. TSA treatment or heat stress result in polyploidy in 2,4-D induced embryonic cells by promoting heterochromatin decondensation.

Fig. 6

a Confocal image of CENH3-GFP-labeled nuclei in protodermal cells at the adaxial side of IZE cotyledons after 9 DOC. IZEs were cultured for 9 days on medium with 2,4-D (left), or after 7 days on 2,4-D medium transferred for two days to medium with 2,4-D and 1 µM TSA (middle), or exposed for 2 days to long heat stress (LHS) at 38 °C (right). b Percentage of polyploid nuclei (based on more than ten detectable CENH3-GFP-labeled centromeres) in cotyledon cells (shown in a). Dots indicate the values of ten biological replicates per treatment with about 200 nuclei analyzed per replicate, bar indicates the mean, and error bars the s.e.m. c Visualization of heterochromatin decondensation in pH2B:H2B-GFP IZEs after 9 DOC on medium containing 2,4-D (left), or after 7 DOC on 2,4-D medium followed by 2 days on 2,4-D and 1 µM TSA (2,4-D + TSA, middle), or by 2 days incubation at 38 °C (2,4D + LSH, right). Size bars indicate 6 µm. a–c Similar results were obtained from two independent experiments. c Numbers in images indicate the frequency with which similar images from different samples were obtained.