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. 2021 Apr 21;12:640932. doi: 10.3389/fmicb.2021.640932

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Copyright © 2021 Rizzo, Da Lio, Panattoni, Salemi, Cappellini, Bartolini and Parrella.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analytical sensitivity of the qPCR SybrGreen assay using the F3/B3 primer pair for ToBRFV detection. Amplification curves in triplicate (A) and the resulting standard curve (B) are shown.