Table 2.
Primers used for conventional RT-PCR and RT-qPCR amplification for comparison with the RT-LAMP method proposed in this study.
| Primers | Sequence (5'–3') | Length (bp) | Annealing (°C) | Protocol | Reference |
|---|---|---|---|---|---|
| ToBRFV-F | AATGTCCATGTTTGTTACGCC | 560 | 58 | RT-PCR end point | Alkowni et al., 2019 |
| ToBRFV-R | CGAATGTGATTTAAAACTGTGAAT | ||||
| ToBRFV_B3 | GGACACCGTCAACTAGGA | 278 | 58 | RT-qPCR SybrGreen | This study |
| ToBRFV_F3 | TTGGAGTCTTAGATGTTGCG | ||||
| ToBRFV-F (5503) | GAAGTCCCGATGTCTGTAAGG | 842 | 55 | RT-PCR end point | Ling et al., 2019 |
| ToBRFV-R (6344) | GTGCCTACGGATGTGTATGA |