Table 3.
Dilutions | RT-LAMP | Visual RT- LAMP | RT-PCR1 | RT-PCR2 | RT-qPCR SybrGreen3 |
---|---|---|---|---|---|
Cq means ± SD | Positive (+)/negative (−) | Positive (+)/negative (−) | Positive (+)/negative (−) | Cq means ± SD | |
22.5 ng/μl | 3.80 ± 0.15 | + | + | + | 5.99 ± 0.34 |
2.25 ng/μl | 5.47 ± 0.11 | + | + | + | 10.05 ± 0.36 |
0.225 ng/μl | 6.57 ± 0.13 | + | + | + | 13.81 ± 1.95 |
22.5 pg/μl | 7.71 ± 0.37 | + | + | + | 18.46 ± 2.20 |
2.25 pg/μl | 8.59 ± 0.07 | + | + | + | 21.86 ± 1.87 |
0.225 pg/μl | 10.34 ± 0.48 | + | + | − | 25.18 ± 2.03 |
22.5 fg/μl | 12.22 ± 0.86 | + | − | − | 28.47 ± 2.14 |
2.25 fg/μl | 16.04 ± 2.75 | + | − | − | 32.43 ± 1.99 |
0.225 fg/μl | n/a4 | − | − | − | n/a |
0.0225 fg/μl | n/a | − | − | − | n/a |
Mean Cq ± SD = mean of the three threshold cycles of each dilution (Cq) ± standard deviation (SD). Cq values above 30 were considered as negative results.
Based on Alkowni et al. (2019).
Based on Ling et al. (2019).
Based on F3 and B3 primer pair.
n/a = not applicable.