Skip to main content
. 2021 Apr 21;12:640932. doi: 10.3389/fmicb.2021.640932

Table 3.

Sensitivity of LAMP used for the ToBRFV detection with different techniques.

Dilutions RT-LAMP Visual RT- LAMP RT-PCR1 RT-PCR2 RT-qPCR SybrGreen3
Cq means ± SD Positive (+)/negative (−) Positive (+)/negative (−) Positive (+)/negative (−) Cq means ± SD
22.5 ng/μl 3.80 ± 0.15 + + + 5.99 ± 0.34
2.25 ng/μl 5.47 ± 0.11 + + + 10.05 ± 0.36
0.225 ng/μl 6.57 ± 0.13 + + + 13.81 ± 1.95
22.5 pg/μl 7.71 ± 0.37 + + + 18.46 ± 2.20
2.25 pg/μl 8.59 ± 0.07 + + + 21.86 ± 1.87
0.225 pg/μl 10.34 ± 0.48 + + 25.18 ± 2.03
22.5 fg/μl 12.22 ± 0.86 + 28.47 ± 2.14
2.25 fg/μl 16.04 ± 2.75 + 32.43 ± 1.99
0.225 fg/μl n/a4 n/a
0.0225 fg/μl n/a n/a

Mean Cq ± SD = mean of the three threshold cycles of each dilution (Cq) ± standard deviation (SD). Cq values above 30 were considered as negative results.

2

Based on Ling et al. (2019).

3

Based on F3 and B3 primer pair.

4

n/a = not applicable.