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. 2021 Apr 21;12:666935. doi: 10.3389/fimmu.2021.666935

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Copyright © 2021 Rindler, Bauer, Jonak, Wielscher, Shaw, Rojahn, Thaler, Porkert, Simonitsch-Klupp, Weninger, Mayerhoefer, Farlik and Brunner

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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scRNA-seq workflow and map of isolated cells from lymph node, blood, and skin. (A) Outline of steps for single cell profiling of trancriptomes from different tissues. (B) UMAP of 9,028 cells integrated from lymph node (1,215), blood (3,301), and skin (4,512) samples according to similarity of their transcriptome, resulting in 19 different color-coded clusters. (C) Unsupervised hierarchical clustering showing relatedness of cell clusters (average gene signatures; correlation distance metric and average linkage). (D) Combined feature plots of all samples showing expression distribution for canonical markers. Intensity of normalized expression for each cell is color-coded (red) and overlaid onto UMAP plots.