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. 2021 May 4;12:2507. doi: 10.1038/s41467-021-22787-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Cells were treated for 5 days with GSI (0.5 μM) and the lysates were immunoblotted for the indicated antibodies. HPB-ALL samples: higher exposure time. The values shown in the right panel are mean ± SEM (n = 3 biologically independent samples per cell line). An unpaired two-sided t test was used to assess the differences in mean protein expression (n = 9 biologically independent samples examined over 3 independent experiments). b Cells were treated with GSI (0.5 μM), sotrastaurin (5 μM) or the combination, and the lysates were immunoblotted for the indicated antibodies. P-S6 and S6: 3 days of treatment; cMyc: 5 days of treatment. JURKAT: the blot is representative of 3 (cMyc) or 4 (S6) independent experiments. MOLT-3/S6: n = 2. MOLT-3/cMyc and PEER: n = 1. c Cells were treated for 6 days with GSI (0.5 μM), sotrastaurin (5 μM) or the combination, and they were assayed for cell survival by CCK8 assay. The values shown are mean ± SEM (JURKAT: n = 8 biologically independent samples examined over 4 independent experiments; MOLT-3 and PEER: n = 4 biologically independent samples examined over two independent experiments). An unpaired two-sided t test was used to assess the differences in mean survival. Bliss >0 synergy; <0 antagonism; =0 additive effect. d Parental and persister DND-41 cells were treated for 5 days with GSI (0.5 μM), sotrastaurin (5 μM) or the combination, and the lysates were immunoblotted for the indicated antibodies (left blot is representative of n = 3 biologically independent experiments; right blot of n = 2). e Cell survival assay of parental and persister DND-41 cells in response to increasing doses of sotrastaurin (5 μM) for 6 days. The values shown are mean ± SEM (n = 12 biologically independent samples examined over three independent experiments). Half-maximal inhibitory concentration (IC50) was calculated using a non-linear regression in GraphPad Prism. An unpaired two-sided t test was used to assess the differences of mean IC-50 (log-transformed values). Protein expression was quantified in ImageJ for all blots. SOTRA sotrastaurin, Ctrl DMSO-treated cells. Source data are provided as Source Data file.