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. 2021 May 4;11:9520. doi: 10.1038/s41598-021-89086-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of MAT on axonal loss in optic nerve. Both sides of optic nerves and the retina were harvested from naive and MAT- or vehicle-treated rats. (A) Detection of NF by immunofluorescence staining in the optic nerve and the retina. ONL, outer nuclear layer; INL: inner nuclear layer; GCL, ganglion cell layer. Scale bars = 100 µm. Quantitative analyses of immunofluorescence in optic nerve (B) and the retina (C) were expressed by average optical density (AOD) of NFs. Data are expressed as mean ± SD (n = 20 optic nerves and the retinas from 10 rats per group). Multiple comparisons were performed using one-way ANOVA, followed by Student–Newman–Keuls test. **P < 0.01, ***P < 0.001, comparison with the naive group. ^##P < 0.01, ^###P < 0.001, comparisons between vehicle- and MAT-treated groups.