FIGURE 3.

The Nsun5 deletion reduces cell proliferation. (A,D) H&E stained sections from E14.5 hearts indicated a thinner ventricular septum resulting from Nsun5 deletion. n = 5/group. (B,E) Quantitative results showed reduced proliferation in Nsun5 deletion OFT aorta area by immunofluorescence staining. The data was presented as the ratio of Ki67⁺ cells/total OFT aorta area cells. Cardiomyocytes were labeled by TnT. n = 5/group. (C,F) Quantitative results showed reduced proliferation in Nsun5 deletion ventricular septum by immunofluorescence staining. The data was presented as the ratio of Ki67⁺ cells/total ventricular septum cells. n = 5/group. Scale bar = 50 or 25 μm. All the data were the mean ± SEMs of three dependent experiments. One-way ANOVA test used for statistical calculation. *P < 0.05, **P < 0.01.