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Manual cell count of L. mexicana cultures grown in the presence or absence of compound 4 and pentamidine at 5 × EC50, with or without wash after 5 or 48 h of incubation. (A) Compound 4. (B) Pentamidine. The dotted brown line indicates the detection limit, being 104 cells/ml. For convenience, where no cells were observed in the counting chamber, the values of 5,000, 4,000, or 3,000 cells/ml were entered to facilitate a graphical representation.
