Figure 4.

SFRP4 regulates adipogenic protein expression in brown adipocytes. (A) Adiponectin and leptin protein expression were measured by western blotting. (B) FABP4 protein expression was measured by western blotting. (C) Quantification of western blot analysis. With SFRP4 treatment, adiponectin protein expression was significantly inhibited in a dose-independent manner, and leptin protein expression was 1.4- and 2.5-fold at concentrations of 10 and 100 ng/ml, respectively, after SFRP4 treatment. Data are expressed as the mean ± SEM. n=4 for each group. ^*P<0.05, ^***P<0.001 vs. control. SFRP4, secreted frizzled related protein 4; FABP4, fatty acid-binding protein 4.