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. 2021 Apr 15;21(6):637. doi: 10.3892/etm.2021.10069

Figure 6.

Figure 6

IL-1β inhibits the mRNA and protein expression of adipogenic markers. (A) C/EBPα, C/EBPβ, Cidea, PPARγ and UCP-1 were assessed by reverse transcription-quantitative PCR. At the concentration of 25 ng/ml IL-1β, C/EBPα, C/EBPβ and PPARγ were not changed compared with the absence of IL-1β. Cidea and UCP-1 mRNA expression levels were all significantly decreased in the presence of IL-1β compared with the absence of IL-1β. (B) Adiponectin, FABP4 and UCP-1 were analysed by western blotting. (C) Quantification of western blotting analysis. With the treatment of IL-1β, adiponectin protein expression was significantly increased with the treatment of IL-1β. UCP-1 protein expression was decreased 0.5-fold compared with the absence of IL-1β. Data are expressed as the mean ± SEM. n=4 for each group. *P<0.05; **P<0.01 vs. control. UCP-1, uncoupling protein 1; C/EBPα/β, CCAAT/enhancer-binding protein α/β; Cidea, cell death-inducing dffa-like effector a; PPARγ, peroxisome proliferator-activated receptor γ; FABP4, fatty acid-binding protein 4.