Table II.
Hematological values of male rats treated with AT-533 or AT-533 gel for 30 days.
| Parameters | Control | Vehicle | 1 mg/kg | 2 mg/kg | 4 mg/kg | Blank gel | AT-533 gel |
|---|---|---|---|---|---|---|---|
| WBC (x109/l) | 5.27±4.29 | 7.34±2.66 | 10.83±3.44 | 12.74±3.92 | 14.45±4.06a | 8.56±1.21 | 11.77±1.55 |
| RBC (x1012/l) | 7.65±1.02 | 8.32±0.51 | 8.10±0.35 | 7.66±0.78 | 7.08±0.22 | 7.89±0.35 | 8.67±0.48 |
| HGB (g/l) | 154.00±6.38 | 160.33±8.12 | 156.83±5.91 | 141.00±13.10a | 130.33±1.53 | 154.43±9.50 | 166.00±7.21a |
| HCT (%) | 41.90±5.31 | 46.72±2.53 | 45.82±1.68 | 40.00±3.27b | 36.83±1.08a | 44.14±2.51 | 46.72±2.07 |
| MCV (fl) | 54.86±1.29 | 56.18±1.64 | 56.58±1.66 | 53.60±3.42 | 52.10±2.91a | 55.94±2.10 | 53.92±2.64 |
| MCH (pg) | 18.36±1.54 | 19.28±0.33 | 19.35±0.38 | 18.47±0.27 | 18.47±0.81 | 19.57±0.75 | 19.18±0.56 |
| MCHC (g/l) | 335.00±30.94 | 343.50±6.09 | 342.33±4.03 | 345.17±18.65 | 354.00±7.00 | 349.86±2.54 | 355.40±10.24 |
| PLT (x109/l) | 1,144.50±78.86 | 1,188.67±104.82 | 1,240.17±88.11 | 1,488.50±114.31c | 1,511.00±109.08c | 1,165.00±69.38 | 1,131.25±138.69 |
| RDW-SD (fl) | 29.26±1.90 | 29.32±1.23 | 30.17±0.83 | 29.30±1.22 | 28.80±1.61 | 29.39±1.32 | 28.48±1.54 |
| RDW-CV (%) | 17.00±1.98 | 17.30±0.87 | 17.35±0.87 | 17.73±1.44 | 16.97±2.60 | 17.19±0.63 | 17.92±1.10 |
| PDW (fl) | 10.42±1.64 | 9.30±0.20 | 9.43±0.27 | 9.55±0.68 | 8.43±0.15 | 9.80±0.77 | 9.86±0.59 |
| MPV (fl) | 8.96±0.57 | 8.33±0.16 | 8.42±0.27 | 8.57±0.39 | 7.73±0.06a | 8.63±0.48 | 8.64±0.42 |
| P-LCR (%) | 18.28±5.03 | 13.35±1.33 | 14.30±1.81 | 15.32±3.08 | 9.33±0.21 | 15.83±3.85 | 15.76±3.22 |
| PCT (%) | 1.00±0.08 | 0.99±0.07 | 1.05±0.06 | 1.28±0.07 | 1.17±0.09 | 0.97±0.06 | 0.91±0.15 |
| NE (x109/l) | 0.71±0.48 | 1.20±0.50 | 2.83±0.71 | 4.45±1.35c | 8.61±1.58c | 2.26±0.59 | 1.90±1.47 |
| LY (x109/l) | 3.70±2.80 | 6.23±2.31 | 6.53±1.76 | 8.19±1.96 | 4.85±2.34 | 6.48±0.61 | 7.99±2.59 |
| MO (x109/l) | 0.12±0.08 | 0.29±0.17 | 0.37±0.16 | 0.81±0.32b | 0.91±0.30c | 0.37±0.15 | 0.54±0.32 |
| EO (x109/l) | 0.08±0.05 | 0.14±0.06 | 0.13±0.07 | 0.08±0.05 | 0.07±0.05 | 0.13±0.03 | 0.09±0.08 |
| BA (x109/l) | 0.00±0.00 | 0.00±0.00 | 0.00±0.00 | 0.01±0.01 | 0.00±0.01 | 0.00±0.00 | 0.01±0.01e |
| NE (%) | 22.68±11.89 | 17.85±5.43 | 27.17±7.74 | 35.63±5.99b | 60.60±5.93c | 19.00±8.57 | 30.93±13.14 |
| LY (%) | 72.32±11.22 | 77.12±5.96 | 68.35±8.16 | 57.48±5.62c | 32.40±6.15c | 76.14±9.76 | 67.60±14.28 |
| MO (%) | 3.16±0.74 | 3.64±1.22 | 3.12±0.58 | 6.23±0.90c | 6.47±2.42b | 3.54±1.07 | 5.24±1.3d |
| EO (%) | 1.84±0.72 | 1.70±0.47 | 1.13±0.35 | 0.68±0.20c | 0.50±0.26c | 1.30±0.34 | 0.92±0.55 |
| BA (%) | 0.00±0.00 | 0.00±0.00 | 0.02±0.04 | 0.05±0.05 | 0.03±0.06 | 0.03±0.05 | 0.16±0.21f |
| RET (x109/l) | 276.10±75.90 | 284.65±52.34 | 269.70±55.99 | 309.68±65.48 | 161.27±6.10a | 301.59±33.05 | 141.52±122.70e |
| RET (%) | 3.56±0.64 | 3.41±0.49 | 3.34±0.74 | 4.58±2.08 | 2.28±0.11 | 3.83±0.42 | 1.03±0.61f |
| PT (s) | 9.74±0.46 | 9.48±0.39 | 9.70±0.34 | 8.75±0.43a | 8.50±0.50b | 9.37±0.26 | 9.72±0.54 |
| TT (s) | 47.74±5.39 | 51.35±7.93 | 51.87±7.05 | 35.62±5.71b | 37.17±6.57b | 48.62±8.52 | 50.50±5.94 |
Values are presented as the mean ± SD for 6 rats in each group (4 rats in the 4 mg/kg group).
aP<0.05,
bP<0.01,
cP<0.001 vs. vehicle.
dP<0.05,
eP<0.001
fP<0.001 vs. blank gel. BA, basophil; EO, eosinophil; HCT, hematocrit; HGB, hemoglobin concentration; LY, lymphocyte; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; MCV, mean corpuscular volume; MO, monocyte; MPV, mean platelet volume; NE, neutrophil; P-LCR, PLT, platelet count; PT, prothrombin time; RBC, erythrocyte count; RDW, red cell distribution width; RET, reticulocyte count; TT, thrombin time; WBC, white blood cell count.