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. Author manuscript; available in PMC: 2021 May 5.
Published in final edited form as: Nat Protoc. 2020 Aug 21;15(9):3105–3128. doi: 10.1038/s41596-020-0372-y

Figure 2. Genotyping for prkdc and il2rgα mutations.

Figure 2.

(A) Schematic of the genotyping experiment using the scale resection approach described in Steps 3–22. (B) Experimental setup for a 96-animal genotyping experiment. (C) Animal is imaged under the dissecting microscope prior to scale resection. Scales are resected from the trunk region by a scrapping downward using a sharp tipped forceps (noted by arrow direction). (D) Same animal following scale resection. Note no visible bleeding was caused by the procedure. (E) Microscopic image of a single resected scale held by the sharp tipped forceps. (F) Qiaxcel DNA screening analysis of PCR amplicons and those digested with Hinfl enzyme (prkdc) and RsaI enzyme (il2rgα). Scale bar = 0.5 cm (C, D), 1 mm (E). Institutional approval from Massachusetts General Hospital was obtained for the experiments shown in this figure.