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. Author manuscript; available in PMC: 2021 May 5.
Published in final edited form as: Nat Protoc. 2020 Aug 21;15(9):3105–3128. doi: 10.1038/s41596-020-0372-y

Figure 3. Acclimating prkdc−/−, il2rgα−/− zebrafish for long term housing at 37 °C.

Figure 3.

(A) Schematic of acclimation process described in Steps 23–25. (B) prkdc−/−, il2rgα−/− zebrafish are moved to a general-purpose water bath initially set at 32 °C. The temperature is then gradually increased to 37 °C at 1 °C/d. The animals can be housed long-term in the 37°C water bath or in a dedicated standalone husbandry unit. In both cases, water needs to be supplemented with antibiotics. (C-E) Representative images of the antibiotics supplemented, heated stand-alone fish system produced by Aquarius Fish Systems. Institutional approval from Massachusetts General Hospital was obtained for the experiments shown in this figure.