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. Author manuscript; available in PMC: 2021 May 5.
Published in final edited form as: Nat Protoc. 2020 Aug 21;15(9):3105–3128. doi: 10.1038/s41596-020-0372-y

Figure 6. Engraftment of EGFP-expressing RD cells into prkdc−/−, il2rgα−/− zebrafish.

Figure 6.

(A) Representative epifluorescence images of prkdc−/−, il2rgα−/− zebrafish engrafted intraperitoneally with EGFP-expressing RD cells at day 0 (left) and day 28 (right). (B) Representative confocal images of prkdc−/−, il2rgα−/− zebrafish engrafted periocularly with EGFP-expressing RD cells at day 0 (left) and day 21 (right). (C, D) Hematoxylin and eosin staining of intraperitoneally (C) and periocularly (D) engrafted EGFP-expressing RD cells. (E-H) Immunohistochemistry staining of intraperitoneally engrafted EGFP-expressing RD cells stained with proliferation marker KI67 (E), apoptosis marker TUNEL (F), as well as clinically relevant histopathology marker MYOD1 (G) and Desmin (H). Scale bar equals 0.5cm (A), 200 μm (B), 5mm (C, D), and 1mm (E-H). Institutional approval from Massachusetts General Hospital was obtained for the experiments shown in this figure.