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. Author manuscript; available in PMC: 2021 May 5.
Published in final edited form as: Nat Protoc. 2020 Aug 21;15(9):3105–3128. doi: 10.1038/s41596-020-0372-y
Reagent 1 Reaction (μl) 1 plate (100 reactions, μl)
10x NEB standard Taq Pol buffer 2.5 250
100 mM dNTP 0.2 20
10 μM Forward prkdc primer 0.5 50
10 μM Reverse prkdc primer 0.5 50
Taq DNA Polymerase 0.1 10
Nuclease-free H2O 18.7 1870
Total 22.5 2250