| Reagent | 1 Reaction (μl) | 1 plate (100 reactions, μl) |
|---|---|---|
| HinfI | 0.3 | 30 |
| NEB Buffer 2.1 or 3.1 or CutSmart | 2 | 200 |
| Nuclease-free water | 2.7 | 270 |
| Total | 5 | 500 |
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