| Denature | Anneal | Extend | Hold | |
|---|---|---|---|---|
| Denature | 94 °C, 2 min | |||
| Cycle 1–36 | 94 °C, 30 s | 60 °C, 30 s | 68 °C, 30 sec | |
| Final extension | 68 °C, 5 min | 4 °C, ∞ |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.