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. 2020 Nov 23;38(5):1744–1760. doi: 10.1093/molbev/msaa304

Fig. 4.

Fig. 4.

Opsin expression varies between life stages and symbiotic state in Aiptasia. (A) Bar chart comparing the expression (TMM normalized reads) of Aiptasia opsins in adults and larvae. ASO-I.2, ASO-II.2, ASO-II.3, ASO-II.5, and ASO-II.12 are significantly upregulated in larva. ASO-II.8, ASO-II.9, and ASO-II.11 are significantly upregulated in adults. (B) Bar chart comparing the expression (TMM normalized reads) of Aiptasia opsins in symbiotic and aposymbiotic adults. ASO-II.12, ASO-II.7, and ASO-II.11 are significantly upregulated in symbiotic adults. (C) Tissue-specific qPCR after Methacarn fixation of adult Aiptasia polyps reveals that ASO-II.3 is significantly upregulated in mesenteries, when compared with tentacle tissue. Data for all other opsins are shown in supplementary figure 3, Supplementary Material online. For all charts significant differences are *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001; and error bars are SEM.