Figure EV2. Responses to 405 and 525 nm light in Lamplight‐expressing retinas were absent in uninjected control.

• A, B
  MEA recordings from uninjected rd1 retina in response to 2 s 405 and 525 nm light. The objective statistical criteria used to identify putative light responsive single units applied to Lamplight retinas (Fig 3) returned 6 units classified as showing increases in firing following 405 nm light. Of these, 2 exhibited a slow and sustained excitation (especially to first stimulus) characteristic of ipRGC responses (A); the remainder did not show a repeatable light response across multiple stimulus presentations, indicating that they were likely false positives (representative in B).
• C
  Mean firing rate for all putative “light responsive” units (N = 6) from uninjected retina. Error bars show SEM.

Data information: All representative retinal single units show perievent rasters (first trial at top) and associated perievent firing rate histograms (Bin size = 500 ms). Timing of light stimuli shown by shaded vertical bars. All light stimuli are 16 log total Lamplight effective photons/cm²/s.