Figure 1. Endothelial IGF‐1R overexpression prevents high‐fat diet (HFD)‐induced weight gain.

• A
  Schematic representation of feeding time course.
• B, C
  In primary endothelial cells isolated from human IGF‐1 receptor endothelial overexpressing mice (hIGFREO) and wild‐type littermates (WT), quantitative polymerase chain reaction (qPCR) shows that hIGFREO have increased expression of human IGF‐1R but similar levels of murine insulin receptor (IR) gene expression as WT (n = 3–5 mice per group).
• D, E
  In primary endothelial cells isolated from WT and hIGFREO, immunoblotting shows that hIGFREO have increased expression of IGF‐1R but similar levels of IR protein expression (n = 3–4 mice per group).
• F
  Chow‐fed hIGFREO had similar body mass to WT; however, hIGFREO did not gain as much weight as WT after 8 weeks of HFD (n = 6–10 mice per group).
• G
  Representative images of difference in fat and water distribution shown by magnetic resonance (MR) imaging in hIGFREO and WT. Scale bar = 1 cm.
• H
  Subcutaneous white adipose tissue (sWAT) and visceral white adipose tissue (vWAT) volumes were reduced in hIGFREO (n = 4 per genotype).
• I
  hIGFREO had reduced white epididymal adipose depot weight compared with WT; there was no difference in heart, spleen or liver weight (n = 7–11 mice per group).
• J
  hIGFREO had similar whole‐body and femur length as WT (n = 7–9 mice per group).

Data information: Data shown as mean ± SEM, individual mice are shown as data points, P < 0.05 taken as being statistically significant using Student’s t‐test and denoted as * (** denotes P ≤ 0.01, ns denotes not significant).

Source data are available online for this figure.