Figure EV1. Endothelial IGF‐1R overexpression has no effect on glucose tolerance in chow‐fed mice.

• A, B
  In aorta from human IGF‐1R endothelial overexpressing mice (hIGFREO) and wild‐type littermates (WT) after 8 weeks HFD, immunoblotting shows that hIGFREO have similar protein expression of phospho‐eNOS at Serine 1177 and phospho‐AKT at Serine 473 (n = 8–10 mice per group).
• C
  hIGFREO had comparable glucose fasting blood glucose levels compared with WT on chow diet (n = 5–10 mice per group).
• D, E
  hIGFREO had comparable glucose intolerance, compared to WT on chow diet (as measured by glucose tolerance test and area under the curve (AUC)) (5–10 mice per group).
• F, G
  In muscle from WT and hIGFREO after 8 weeks of HFD, immunoblotting shows that hIGFREO have increased protein expression of total and phospho‐AKT at Serine 473 (n = 3 per genotype).

Data information: Data shown as mean ± SEM and individual mice are shown as data points, P < 0.05 taken as being statistically significant using Student’s t‐test. ns denotes not significant.

Source data are available online for this figure.