Figure EV2. Protection from high‐fat diet (HFD)‐induced weight gain is not due to altered energy expenditure in mice overexpressing human IGF‐1R in the endothelium (hIGFREO).

• A–C
  hIGFREO exhibit no difference in oxygen consumption, CO₂ production or respiratory exchange ratio using indirect calorimeter assessment after HFD when compared to WT after 8 weeks of HFD (n = 4 per genotype).
• D, E
  hIGFREO had comparable concentrations of fasting plasma leptin and adiponectin to WT after 8 weeks of HFD (n = 5–7 mice per group).

Data information: Data shown as mean ± SEM and individual mice are shown as data points, P < 0.05 taken as being statistically significant using Student’s t‐test and denoted as * and ns denotes not significant. For indirect calorimetry, ANOVA testing was performed using mass as a co‐variant (ANCOVA testing) using calrapp.org.