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. 2021 May 2;22(5):e50767. doi: 10.15252/embr.202050767

Figure 3. Protection from high‐fat diet (HFD)‐induced weight gain in human IGF‐1R endothelial overexpressing mice (hIGFREO) is not due to changes in activity, food intake, energy expenditure, adipose browning or gut transit time.

Figure 3

  • A–C
    hIGFREO exhibit no difference in activity levels, food consumption or energy expenditure using indirect calorimeter assessment after HFD compared with wild‐type littermates (WT) after HFD. (n = 4 per genotype).
  • D
    Adipose expression of browning markers is also no different in white epididymal adipose tissue and brown adipose tissue compared with WT (n = 6 per genotype).
  • E
    Core body temperature is no different in hIGFREO compared with WT (n = 6–8 mice per group).
  • F, G
    Gut transit time is also unaltered in hIGFREO compared with WT as shown by no change in small intestine length (F) (n = 7–9 mice per group), or total gut transit time after a carmine red gavage (G) (n = 12–13 mice per group).

Data information: The light/dark cycle for graphs A–C is shown as follows: light in yellow and dark in brown. Data shown as mean ± SEM and individual mice are shown as data points. For indirect calorimetry, ANOVA testing was performed using mass as a co‐variant (ANCOVA testing) using calrapp.org. ns denotes not significant.