Figure EV4. There was no difference in hepatic steatosis in human IGF‐1 receptor endothelial overexpressing mice (hIGFREO) in the setting of high‐fat diet‐induced obesity.

• A
  Representative images of haematoxylin‐ and eosin‐stained liver from hIGFREO compared with wild‐type littermates (WT). Scale bar = 200 µm.
• B
  No difference in extent of hepatic fibrosis in hIGFREO compared with WT after 8 weeks of HFD (n = 7–9 mice per group).
• C
  This was confirmed using MR images showing that there was no difference in hepatic fat content when comparing hIGFREO to WT after 8 weeks of HFD (n = 4 per genotype).
• D–F
  Hepatic levels of free fatty acids, triglycerides and cholesterol are similar when comparing hIGFREO to WT after 8 weeks of HFD (n = 7–11 mice per group).
• G, H
  Fasted plasma concentration of free fatty acids and triglycerides is also similar in hIGFREO and WT (n = 4–8 mice per group).
• I, J
  (I) Pancreatic lipase levels and lipase activity (J) were similar in hIGFREO and WT after 8 weeks of HFD (n = 4–6 mice per group).
• K
  Hepatic expression of CYP7A and ABCB11 is also similar in hIGFREO and WT after 8 weeks of HFD (n = 5–6 mice per group).

Data information: Data shown as mean ± SEM and individual mice are shown as data points, P < 0.05 taken as being statistically significant using Student’s t‐test. ns denotes not significant.