Reagent/Resource	Reference or Source	Identifier or Catalog Number
Experimental Models
HeLa‐Kyoto cell line	Poser et al (2008)	N/A
HeLa‐Kyoto G3BP2‐GFP bac cell line	Poser et al (2008)	N/A
HeLa‐Kyoto GFP‐FUS WT bac cell line	Mateju et al (2017)	N/A
HeLa‐Kyoto GFP‐FUS G156E bac cell line	Mateju et al (2017)	N/A
HeLa‐Kyoto mcherry‐G3BP1 bac cell line	Poser et al (2008)	N/A
HEK293T PABPC1‐Dendra‐2	This paper	N/A
HeLa Flp‐In T‐REx V5‐HSP70	This paper	N/A
Recombinant DNA
eGFP	Clontech Laboratories, Inc.	Contact company
pcDNA5/FRT/TO GFP	Hageman and Kampinga (2009)	N/A
GFP‐DYRK3	Wippich et al (2013)	N/A
GFP‐DYRK3‐ΔNT	Wippich et al (2013)	N/A
Sup35‐NM‐GFP‐DYRK3‐dN	This study	N/A
mcherry‐HSP90α	Lev et al (2008)	N/A
mcherry‐HSP90β	Picard et al (2006)	N/A
myc‐RAPTOR	Wippich et al (2013)	N/A
mRFP‐DCP1A	Kedersha et al (2008)	N/A
pcDNA5/FRT‐TO‐V5‐HSP70	Hageman and Kampinga (2009)	N/A
Antibodies
DYRK3	Aviva‐System Biology	ARP30647_P050
Fibrillarin	EnCor Biotechnology Inc.	MCA‐38F3
GFP	Clontech	632375
G3BP	BD bioscience	611127
HSPA1A (Hsp70/Hsc70)	StressMarq Biosciences	SMC‐104A
HSPA8 (Hsc70/Hsp73)	StressMarq Biosciences	SMC‐151A
HSP90 total	StressMarq Biosciences	SMC‐149
HSP90 alpha	StressMarq Biosciences	SMC‐147A
HSP90 beta	StressMarq Biosciences	SMC‐107A
myc	Santa Cruz Biotechnology	SC‐40
Phospho‐PRAS40 (Thr246)	Cell Signaling	13175
Phospho‐p70 S6 Kinase (Thr389)	Cell Signaling	9205
Phospho‐ser10‐H3	Activemotif	39253
Phospho‐4E‐BP1 (Thr37/46)	Cell Signaling	2855
p70 S6 Kinase	Cell Signaling	9202
PRAS40	Cell Signaling	2610
Puromycin (Clone 12D10)	Merck	MABE343
SC‐35	Sigma‐Aldrich	S4015
TIA‐1	Santa Cruz Biotechnology	SC‐1751
TUBA4A	Sigma‐Aldrich	T6074
Ubiquitin	Dako	Z0458
4E‐BP1	Cell Signaling	9644
Alexa Fluor™ 594 Azide	Thermo Scientific	A‐10270
Donkey anti‐Mouse IgG (H + L), Alexa Fluor® 594	Thermo Scientific	A‐21203
Donkey anti‐Mouse IgG (H + L), Alexa Fluor® 488	Thermo Scientific	A‐21202
Donkey anti‐Mouse IgG (H + L), Alexa Fluor® 647	Thermo Scientific	A‐31571
Donkey anti‐Rabbit IgG (H + L), Alexa Fluor® 488	Thermo Scientific	A‐21206
MOUSE IGG HRP LINKED WHOLE AB	GE Healthcare	NXA931
RABBIT IGG HRP LINKED WHOLE AB	GE Healthcare	NA934
Oligonucleotides and other sequence‐based reagents
ON‐TARGETplus Non‐targeting siRNA #1	Dharmacon	Contact company
ON‐TARGETplus human HSP90α siRNA	Dharmacon	Contact company
ON‐TARGETplus human HSP90β siRNA	Dharmacon	Contact company
Chemicals, Enzymes and other reagents
Ammonium Chloride	Sigma‐Aldrich	254134
Blasticidin S HCl	Gibco	12172530
Complete‐EDTA	Roche	11873580001
DAPI	Santa Cruz Biotechnology	SC3598
Duolink™ In Situ Red Starter Kit Mouse/Rabbit	Sigma‐Aldrich	DUO92101
Geldanamycin	Enzo Life Science	BML‐EI280
GFP‐Trap® Agarose beads	Chromotek	Gta‐20
GSK626616	BioVision	B2452
G418‐Geneticin	Aurogene	L0015
Hygromycin B	Sigma‐Aldrich	H0654
Ly294002	Sigma‐Aldrich	L9908
O‐Propargyl‐puromycin (OP‐puro)	Jena Bioscience	NU‐931‐05
Puromycin dihydrochloride	Sigma‐Aldrich	P8833
Sodium Arsenite	Carlo Erba	S7400
Sodium Arsenite	Sigma‐Aldrich	106277
Tetracycline hydrochloride	Sigma‐Aldrich	T7660
VER‐155008	Sigma‐Aldrich	SML0271
Wortmannin	Sigma‐Aldrich	W1628
Z‐Leu‐Leu‐Leu‐al (MG132)	Sigma‐Aldrich	C2211
17‐AAG	Enzo Life Science	BML‐EI308
Software
Daniel's XL Toolbox	open‐source add‐in for Microsoft® Excel®	https://www.xltoolbox.net/
Fiji	NIH	https://fiji.sc/
GraphPad Prism6 software	GraphPad	https://www.graphpad.com
ScanR Olympus analysis software	Olympus	https://www.olympus‐lifescience.com
Other