| Reagent/Resource | Reference or Source | Identifier or Catalog Number |
|---|---|---|
|
Experimental Models | ||
| HeLa‐Kyoto cell line | Poser et al (2008) | N/A |
| HeLa‐Kyoto G3BP2‐GFP bac cell line | Poser et al (2008) | N/A |
| HeLa‐Kyoto GFP‐FUS WT bac cell line | Mateju et al (2017) | N/A |
| HeLa‐Kyoto GFP‐FUS G156E bac cell line | Mateju et al (2017) | N/A |
| HeLa‐Kyoto mcherry‐G3BP1 bac cell line | Poser et al (2008) | N/A |
| HEK293T PABPC1‐Dendra‐2 | This paper | N/A |
| HeLa Flp‐In T‐REx V5‐HSP70 | This paper | N/A |
|
Recombinant DNA | ||
| eGFP | Clontech Laboratories, Inc. | Contact company |
| pcDNA5/FRT/TO GFP | Hageman and Kampinga (2009) | N/A |
| GFP‐DYRK3 | Wippich et al (2013) | N/A |
| GFP‐DYRK3‐ΔNT | Wippich et al (2013) | N/A |
| Sup35‐NM‐GFP‐DYRK3‐dN | This study | N/A |
| mcherry‐HSP90α | Lev et al (2008) | N/A |
| mcherry‐HSP90β | Picard et al (2006) | N/A |
| myc‐RAPTOR | Wippich et al (2013) | N/A |
| mRFP‐DCP1A | Kedersha et al (2008) | N/A |
| pcDNA5/FRT‐TO‐V5‐HSP70 | Hageman and Kampinga (2009) | N/A |
|
Antibodies | ||
| DYRK3 | Aviva‐System Biology | ARP30647_P050 |
| Fibrillarin | EnCor Biotechnology Inc. | MCA‐38F3 |
| GFP | Clontech | 632375 |
| G3BP | BD bioscience | 611127 |
| HSPA1A (Hsp70/Hsc70) | StressMarq Biosciences | SMC‐104A |
| HSPA8 (Hsc70/Hsp73) | StressMarq Biosciences | SMC‐151A |
| HSP90 total | StressMarq Biosciences | SMC‐149 |
| HSP90 alpha | StressMarq Biosciences | SMC‐147A |
| HSP90 beta | StressMarq Biosciences | SMC‐107A |
| myc | Santa Cruz Biotechnology | SC‐40 |
| Phospho‐PRAS40 (Thr246) | Cell Signaling | 13175 |
| Phospho‐p70 S6 Kinase (Thr389) | Cell Signaling | 9205 |
| Phospho‐ser10‐H3 | Activemotif | 39253 |
| Phospho‐4E‐BP1 (Thr37/46) | Cell Signaling | 2855 |
| p70 S6 Kinase | Cell Signaling | 9202 |
| PRAS40 | Cell Signaling | 2610 |
| Puromycin (Clone 12D10) | Merck | MABE343 |
| SC‐35 | Sigma‐Aldrich | S4015 |
| TIA‐1 | Santa Cruz Biotechnology | SC‐1751 |
| TUBA4A | Sigma‐Aldrich | T6074 |
| Ubiquitin | Dako | Z0458 |
| 4E‐BP1 | Cell Signaling | 9644 |
| Alexa Fluor™ 594 Azide | Thermo Scientific | A‐10270 |
| Donkey anti‐Mouse IgG (H + L), Alexa Fluor® 594 | Thermo Scientific | A‐21203 |
| Donkey anti‐Mouse IgG (H + L), Alexa Fluor® 488 | Thermo Scientific | A‐21202 |
| Donkey anti‐Mouse IgG (H + L), Alexa Fluor® 647 | Thermo Scientific | A‐31571 |
| Donkey anti‐Rabbit IgG (H + L), Alexa Fluor® 488 | Thermo Scientific | A‐21206 |
| MOUSE IGG HRP LINKED WHOLE AB | GE Healthcare | NXA931 |
| RABBIT IGG HRP LINKED WHOLE AB | GE Healthcare | NA934 |
|
Oligonucleotides and other sequence‐based reagents | ||
| ON‐TARGETplus Non‐targeting siRNA #1 | Dharmacon | Contact company |
| ON‐TARGETplus human HSP90α siRNA | Dharmacon | Contact company |
| ON‐TARGETplus human HSP90β siRNA | Dharmacon | Contact company |
| Chemicals, Enzymes and other reagents | ||
| Ammonium Chloride | Sigma‐Aldrich | 254134 |
| Blasticidin S HCl | Gibco | 12172530 |
| Complete‐EDTA | Roche | 11873580001 |
| DAPI | Santa Cruz Biotechnology | SC3598 |
| Duolink™ In Situ Red Starter Kit Mouse/Rabbit | Sigma‐Aldrich | DUO92101 |
| Geldanamycin | Enzo Life Science | BML‐EI280 |
| GFP‐Trap® Agarose beads | Chromotek | Gta‐20 |
| GSK626616 | BioVision | B2452 |
| G418‐Geneticin | Aurogene | L0015 |
| Hygromycin B | Sigma‐Aldrich | H0654 |
| Ly294002 | Sigma‐Aldrich | L9908 |
| O‐Propargyl‐puromycin (OP‐puro) | Jena Bioscience | NU‐931‐05 |
| Puromycin dihydrochloride | Sigma‐Aldrich | P8833 |
| Sodium Arsenite | Carlo Erba | S7400 |
| Sodium Arsenite | Sigma‐Aldrich | 106277 |
| Tetracycline hydrochloride | Sigma‐Aldrich | T7660 |
| VER‐155008 | Sigma‐Aldrich | SML0271 |
| Wortmannin | Sigma‐Aldrich | W1628 |
| Z‐Leu‐Leu‐Leu‐al (MG132) | Sigma‐Aldrich | C2211 |
| 17‐AAG | Enzo Life Science | BML‐EI308 |
|
Software | ||
| Daniel's XL Toolbox | open‐source add‐in for Microsoft® Excel® | https://www.xltoolbox.net/ |
| Fiji | NIH | https://fiji.sc/ |
| GraphPad Prism6 software | GraphPad | https://www.graphpad.com |
| ScanR Olympus analysis software | Olympus | https://www.olympus‐lifescience.com |
|
Other | ||