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. 2021 Mar 18;22(5):e51280. doi: 10.15252/embr.202051280

Figure EV3. Tlr4 −/− HEI‐OC1 cells show diminished LPS‐responsiveness unless complemented with Tlr4 .

Figure EV3

  1. Comparison of genomic DNA at the Tlr4 locus from Tlr4 −/− HEI‐OC1 and wild‐type cells. Sequences from the Tlr4 −/− cell line contained a single nucleotide insertion or four nucleotide deletion and summarized below.
  2. Anti‐TLR4 staining in Tlr4 −/− and control HEI‐OC1 cells. Bars (lower right) are 50 µm.
  3. Flow cytometric analysis of conjugated LPS internalization in Tlr4 −/− and control HEI‐OC1 cells (n = 4 independent biological replicates).
  4. IL‐6 secretion in Tlr4 −/− and control HEI‐OC1 cells transfected with empty vector (EV), Tlr4 (pTlr4), or left untransfected (−) and subsequently treated with 100 ng/ml LPS (n = 4 independent biological replicates). Inset, fold induction of IL‐6 secretion was determined relative to the untransfected cells treated with LPS.

Data information: In (C and D), data are presented as mean and standard deviation. Statistical comparisons were assessed by 2‐way ANOVA (D). **P < 0.01; ****P < 0.0001 (unpaired Student’s t‐test, C; Bonferroni test, D).

Source data are available online for this figure.