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. 2021 Mar 29;22(5):e51573. doi: 10.15252/embr.202051573

Figure 2. Wound‐induced tumour formation comprises a fibrotic response.

Figure 2

  1. Immunofluorescent images of skin sections from PDGFRα‐eGFP reporter mice with normal (WT, n = 4), inflamed (InvEE, n = 4), wounded (d14 post‐wounding; wound, n = 4) or tumour‐associated (Papilloma, Pap d17 and d30 post‐wounding, n = 4 per condition) skin. Nuclei were stained with Dapi (4’, 6‐diamidino‐2‐phenylindole; blue). Dotted line represents epidermal–dermal boundary. Scale bars: 50 µm.
  2. Quantification of the number of fibroblasts present in different skin conditions (n = 4 mice per condition; **P < 0.01; ***P < 0.001; ****P < 0.0001; One‐way ANOVA testing). Data represent means of multiple microscopic fields ± SEM.
  3. Herovici staining of sections of normal (WT), inflamed (InvEE), wound or tumour‐associated (Papilloma) skin. Immature collagen is stained blue and mature collagen is stained purple. Scale bars: 100 µm.
  4. pNA (p‐nitroaniline) release assessed by spectrophotometric analysis as a measure of serine protease activity in normal (WT, n = 5), inflamed (InvEE, n = 5) and tumour‐associated (Pap, n = 7) skin. (*P < 0.05; one‐way ANOVA testing). Data represent means ± SEM.