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. 2021 Mar 29;22(5):e51573. doi: 10.15252/embr.202051573

Figure EV3. PRSS35 does not alter proliferation or inflammatory responses in fibroblasts.

Figure EV3

  • A
    Cell proliferation assay on PRSS35 WT and PRSS35 KO primary fibroblasts, as measured through live cell imaging (n = 3 biological replicates per condition, ns, Wilcoxon matched‐paired rank test). Data represent means ± SEM
  • B
    Scratch wound assay of PRSS35 WT and PRSS35 KO primary fibroblasts. Scratch width was measured over time with live cell imaging (n = 3 per condition, ns, Wilcoxon matched‐paired rank test). Data represent means ± SEM
  • C, D
    Relative RNA expression of the indicated genes in primary fibroblast isolated from WT and PRSS35 KO mice, untreated or after 24 h stimulation with 10 ng/ml TGF‐β (n = 6 per genotype) or 20 ng/ml IL‐1β (WT, n = 5; PRSS35 KO, n = 6) (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; two‐way ANOVA with multiple comparisons). Data represent means ± SEM.