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. 2021 Feb 18;8(9):2004635. doi: 10.1002/advs.202004635

Figure 3.

Figure 3

KDM5C represses type I interferons (IFNs) and interferon‐stimulating genes (ISGs). A) GO (gene ontology) analysis for genes repressed by KDM5C in MCF7 cells (n = 1658) (fold change (FC) (siKDM5C/siCTL) ≥ 1.5). Top enriched GO terms were shown. B,C) Heat map (B) and box plot (C) representation of the expression (FPKM, log2) for genes repressed by KDM5C (n = 1658). D,E) Genome browser views of representative genes repressed by KDM5C, such as OAS1 (D) and STAT1 (E), were shown. F) MCF7 cells were transfected with siCTL or siKDM5C for three days, followed by RNA extraction and RT‐qPCR analysis to examine the expression of selected type I IFNs and ISGs as indicated. G) MCF7 cells were infected with shCTL or shKDM5C lenti‐virus for three days, followed by RNA extraction and RT‐qPCR analysis to examine the expression of selected type I IFNs and ISGs as indicated. H) Wild type (WT) and KDM5C knockdown (KDM5C (sgRNA)) MCF7 cells were subjected to RNA extraction and RT‐qPCR analysis to examine the expression of selected type I IFNs and ISGs as indicated. I,J) Tumor samples as described in Figure 1L (I) or Figure 1N (J) were subjected to RNA extraction and RT‐qPCR analysis to examine the expression of selected type I IFNs and ISGs as indicated (± SD, * p < 0.05, ** p < 0.01, *** p < 0.001). K,L) Tumor samples as described in Figure 1N were subjected to RNA extraction and RT‐qPCR analysis (K) or immunohistochemistry (L) to examine the expression of Cd8a (± SD, *** p < 0.001).