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. 2021 Feb 25;8(9):2003535. doi: 10.1002/advs.202003535

Figure 2.

Figure 2

MgFe‐LDH is superior to MgAl‐LDH in supporting the pluripotency of mESCs. A) The embryoid bodies differentiated from P1 mESCs treated with nanoparticles at day eight of differentiation. B) The relative mRNA expression of three germ layer‐related genes during embryoid bodies differentiation at d8 was measured by qPCR: Nestin and Sox1 in the ectoderm; Kdr, Emoes, and α‐SMA in the mesoderm; as well as Gata4, Gata6, and Cxcr4 in the endoderm. * represents p < 0.05, when compared to the MgAl‐LDH treatment. C) The embryoid bodies were transferred to a gelatin precoated confocal dish for differentiation for another 4 d following which the presence and localization of NESTIN (ectoderm), α‐SMA (mesoderm), and AFP (endoderm) were determined by immunofluorescence. D) Teratoma formation assay for assessing pluripotency of P3 mESCs treated with nanoparticles. E) Histological analysis by HE staining of teratomas tissues derived from P3 mESCs treated with nanoparticles, to analyze the morphological features of the three germ layers. Arrowheads indicate these morphological features. F) Immunohistochemistry analysis of teratomas sections.