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. 2021 Apr 6;22(5):e51532. doi: 10.15252/embr.202051532

Figure 1. MICU1 deficiency suppresses cold stress‐induced ferroptosis.

Figure 1

  • A
    Schematic model showing the design of the screening.
  • B
    MICU1 was identified using the MAGeCK program with a robust ranking aggregation (RRA) score.
  • C
    Immunoblots of endogenous MICU1 for validating gene knockout. *Nonspecific signals.
  • D
    LDH assay was performed after 24 h of cold stress on ice. Data are presented as mean ± SEM; n = 3, biological replicates. ****P < 0.0001, ***P < 0.001 by one‐way ANOVA followed by Dunnett’s multiple comparison test.
  • E,F
    Lipid peroxidation was measured by C11‐BODIPY 581/591 after cold stress for 5 h. Representative plots are shown in (E), and the data (F) are presented as mean ± SEM; n = 4–6, biological replicates. ****P < 0.0001, *** P < 0.001 by two‐way ANOVA followed by Tukey’s multiple comparison test

Source data are available online for this figure.