Figure S12.

Experimental effect of LIN on channel opening and closing kinetics. Top: Representative effect of LIN on channel opening and closing kinetics. Experiments to determine tau for channel opening were performed by stepping from a holding voltage of −80 mV to indicated test voltages. Experiments to determine tau for channel closing were performed in high potassium solution (100 mM K⁺) by stepping from a test voltage of +40 mV to indicated test voltages. Tau was determined by fitting a single exponential function to the first second following capacitive currents. Bottom: Summary of LIN effects on closing and opening kinetics at indicated voltages. Data shown as mean ± SEM; n = 12. Statistics denote one-sample t test compared with a hypothetical value of 1. **, P < 0.01; ***, P < 0.001. Ctrl, control.