FIG 3.

Deletion of sltA causes multidrug sensitivity to azoles, terbinafine, and simvastatin but not amphotericin B or caspofungin. (A) Equal series numbers of conidia (2 × 10⁴, 2 × 10³, and 2 × 10²) from indicated strains inoculated on YAG medium without drug at 37°C for 1.5 days or with drug at 37°C for 2 days. The following antifungal drugs were used: 0.05 μg ml⁻¹ of itraconazole, 0.1 μg ml⁻¹ of voriconazole, 0.6 μg ml⁻¹ of bifonazole, 200 μg ml⁻¹ of fluconazole, 24 μg ml⁻¹ of simvastatin, 0.2 μg ml⁻¹ of terbinafine, 8 μg ml⁻¹ of amphotericin B, and 0.6 μg ml⁻¹ of caspofungin. (B) MIC Etest strips impregnated with a gradient of itraconazole (ITZ) or voriconazole (VRC) were placed onto YAG agar plates containing a lawn of conidia cultured for 36 h (the upper) or 48 h (the bottom) at 37°C before observation.