FIG 7.

sltA gene deletion in laboratory-derived and clinical azole-resistant strains attenuates the degree of azole resistance. (A) Colony morphologies of the indicated strains (2 × 10⁴, 2 × 10³, and 2 × 10² conidia) grown on YAG medium at 37°C for 36 h with or without 0.2 μg ml⁻¹ of itraconazole. (B) qRT-PCR analysis was performed after growth of cultures in MM for 18 h at 37°C and subsequently for 2 h of treatment with 0.5 μg ml⁻¹ of itraconazole. The tubA gene was used as an internal control. Samples were assessed in biological triplicates. P values were calculated using unpaired t test with Welch’s correction: *, P < 0.05; **, P < 0.01; ***, P < 0.001.