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(A) Immunoblot of IMP variants investigated (IMP-1, -3, -6, -10, -25, -30, -34, -40, -42, -52, and -60). (B) Immunoblot of IMP variants investigated (IMP-61, -66, -70, -76, -77, -78, -79, -80, and -A). Both blots were prepared and exposed at the same time under the same conditions. DnaK was used as a constitutively expressed control. The IMP-60 variant was expressed at a lower level than the others. Low level expression of IMP-60 was further verified by RT-PCR (Fig. S5).
