FIG 2.

Formation and intracellular half-life of AT-9010 in primary human bronchial and nasal epithelial cells. After cells were exposed to 10 μM AT-511 for 8 h, they were rinsed, and fresh medium was applied for 0, 15, 24, 48, and 72 h post-drug removal. Cells were collected and extracted at each time point (n = 3), the active TP was measured by LC-MS/MS, and the half-life (t_1/2) was determined as described in Materials and Methods. Data are expressed at means ± SEs.