Effect of a 30-min treatment with the individual enzymes on the biomass of ATCC 33591, ATCC 35984, and ATCC 47076 biofilms and comparison to the TEC. Hexosaminidase, β-N-acetylhexosaminidase; endonuclease, DNA/RNA endonuclease; endoglucanase, endo-1,4-β-d-glucanase. Data expressed as percentage of the control. The values indicate the means of triplicate experiments (± standard error). Statistical analysis, two-way ANOVA followed by Holm-Sidàk post hoc test. NS, no significant difference; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.