FIG 4.

Effect of the combination of the TEC with antibiotics on MRSE ATCC biofilms. (A) CFU counts. (B) Biomass. (C) Fluorescence microscopy. (A and B) Blue, controls; red, samples treated for 30 min with TEC. T0, no reincubation; TGN, 24 h reincubation in TGN; others, 24 h reincubation in TGN with antibiotics at the indicated concentrations (see Table 1). CFU counts expressed in log₁₀ CFU per coupon, and biomass expressed in percentage of T0 controls. The values indicate the means of triplicate experiments (± standard error). Statistical analysis, two-way ANOVA followed by Tukey HSD comparing control samples to samples exposed to the enzymatic cocktail. NS, no significant difference; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. (C) Green, living cells (SYTO 9); red, dead cells (propidium iodide). ×20 magnification. Scale bars represent 50 μm. VAN, vancomycin; RIF, rifampin; LZD, linezolid; MXF, moxifloxacin; DAP, daptomycin; DOX, doxycycline.