TABLE 1.
MIC profiles for parental strains and transformants (mg/liter)a
| Strain | MIC (mg/liter)b |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TIGb (+ NMP, + PAβN) | ERV (+ NMP, + PAβN) | DOX (+ NMP, + PAβN) | TET (+ NMP, + PAβN) | MIN | OXY | CTC | CTX | CAZ | CQM | FEP | CIP | OLA | |
| Raoultella ornithinolytica NC189c | 4 (0.06, 1) | 2 (0.06,0.5) | 2 (0.125, 0.5) | 2 (0.125, 1) | 2 | 4 | 16 | >128 | >128 | >128 | 16 | 0.03 | 16 |
| E. coli DH5α | 0.25 | 0.5 | 1 | 0.5 | 2 | 2 | 4 | 0.03 | 0.125 | 0.03 | 0.03 | 0.002 | 1 |
| E. coli DH5α/pHSG575 | 0.25 (0.06, 0.125) | 0.5 (0.06, 0.125) | 1 (0.125, 0.25) | 0.5 (0.125, 0.125) | 2 | 4 | 4 | 0.03 | 0.125 | 0.03 | 0.03 | 0.002 | 1 |
| E. coli DH5α/pHSG575-tmexCD2 | 0.5 | 1 | 2 | 1 | 2 | 4 | 8 | 0.06 | 0.125 | 0.125 | 0.06 | 0.008 | 4 |
| E. coli DH5α/pHSG575-tmexCD2-toprJ2 | 2 (0.06, 0.5) | 2 (0.06, 0.5) | 8 (0.125, 0.5) | 4 (0.125, 1) | 4 | 8 | 16 | 0.125 | 0.25 | 0.25 | 0.125 | 0.015 | 4 |
a
Abbreviations: TIG, tigecycline; ERV, eravacycline; DOX, doxycycline; TET, tetracycline; MIN, minocycline; OXY, oxytetracycline; CTC, chlortetracycline; CTX, cefotaxime; CAZ, ceftazidime; CQM, cefquinome; FEP, cefepime; CIP, ciprofloxacin; OLA, olaquindox; NMP, 1-(1-naphthylmethyl)-piperazine; PAβN, phenylalanine arginine β-naphthylamide.
b
MICs of tigecycline were determined by microdilution and those of other antibiotics by agar dilution. The MICs of tigecycline, eravacycline, doxycycline, and tetracycline were also determined with NMP (100 mg/liter) or PAβN (50 mg/liter).
c
The imipenem MIC against NC189 was 4 mg/liter.