FIG 2.

[³H] GGPP incorporation into ubiquinones. (a) RP-HPLC metabolic profiles of erythrocytes uninfected or infected with P. falciparum schizonts. Uninfected or infected erythrocytes were incubated with [³H] GGPP. (b and c) UQ-8 (b) or UQ-9 (c) from [³H] GGPP-radiolabeled parasites were purified by RP-HPLC. Both UQ homologs were chemically reduced and analyzed by the same chromatography method. In all experiments, samples were co-injected with UQ standards. The fractions were collected at 1 min. This experiment was performed twice with similar results. UQ-8, ubiquinone-8; UQH₂-8, ubiquinol-8; UQ-9, ubiquinone-9; UQH₂-9, ubiquinol-9.