FIG 6.
Effects of atovaquone on ubiquinone redox state and atovaquone rescue assays. (a) RP-HPLC of UQ and UQH2 labeled with [3H] GGPP in AV untreated/treated parasites. The fractions were collected at 0.75 min. This experiment was performed three times with similar results. UQ-8, ubiquinone-8; UQH2-8, ubiquinol-8; UQ-9, ubiquinone-9; UQH2-9, ubiquinol-9. (b and c) IC50 values of AV in the presence or absence of 10 μM or 5 μM ubiquinone 10 (UQ-10) or 5 μM decyl-UQ (dUQ) (mean of three identical experiments). The results were studied by unpaired t test with respect to the control. *, P < 0.05, **, P < 0.01, ***, P < 0.001.