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. 2021 Mar 18;65(4):e02095-20. doi: 10.1128/AAC.02095-20

FIG 3.

FIG 3

Adjunct therapy with AX20017 lowers drug tolerance inside murine macrophages. (a) Schematic representation of the peritoneal macrophage infection experiment. (b and c) Peritoneal macrophages were infected with Rv, RvΔG, and RvΔG::G at a multiplicity of infection (MOI) of 1:10. After 4 h, 0.5 μg/ml INH (b) or RIF (c) was added to the infected macrophages. At 48 h postinfection, macrophage cells were lysed, and bacillary survival was examined. CFU obtained for untreated infected macrophages at 48 h postinfection were normalized to 100%. The mean percent intracellular survival (±SD) (n = 3) of the bacterial strain in the treated macrophages was calculated with respect to (w.r.t) the corresponding untreated cells for that strain. (d to g) The same experiment as in panels b and c except with an additional Rv-infected sample, wherein 25 μM AX was added at 4 h postinfection (Rv+AX). Infected macrophages were either left untreated (d) or treated with 0.5 μg/ml INH (e), 0.5 μg/ml RIF (f), or 1 μg/ml BDQ (g). The mean percent intracellular survival (±SD) (n = 3) of the bacterial strain in the treated macrophages was calculated with respect to the corresponding untreated cells for that strain. Data are representative of results from two biologically independent experiments, each performed in triplicates. *, P < 0.05; **, P < 0.005; ***, P < 0.0005.