FIG 5.

PknG is required for the resuscitation of Mtb in the modified Cornell mouse model of infection. (a) Schematic representation of the modified Cornell mouse model of tuberculosis infection experiment. Mice (n = 6 to 10 per group for each time point/strain) were infected with Rv, RvΔG, and RvΔG::G. After the establishment of infection for 4 weeks, antibiotics (INH and RIF) were added to drinking water for 12 weeks to eliminate actively replicating bacilli. Following 4 weeks of rest, dexamethasone (Dx) was injected for 4 weeks for immune suppression. Bacillary survival was examined at day 1 and weeks 4, 16, 30, and 34. (b and c) Bacillary loads in lungs (b) and spleen (c) of infected mice at the indicated time points postinfection (n = 6 to 10). Each data point represents log₁₀ CFU of an infected animal in individual organs, and the error bar depicts the SD for each group. *, P < 0.05; **, P < 0.005; ***, P < 0.0005. N.D., not determined. (d) Table depicting mean log₁₀ CFU ± SD in the lungs and spleen of infected animals at the indicated time points.