FIG 1.

Workflow and optimization. (A) Scheme of anti-Candida auris susceptibility assay. Candida auris harvested from colonies grown on Sabouraud dextrose agar (SDA) were incubated in RPMI medium with compounds to identify inhibitors. (B and C) Optimization of the ATP-based antifungal susceptibility assay by fungal density and incubation time. Cells were diluted with fresh medium into ratios of 1 to 1,000, 1 to 500, and 1 to 200 prior to dispensing into assay plates. (B) Fungal growth was detected by an ATP-based assay at 24 and 48 h. (C) Two known compounds, posaconazole and micafungin, were tested against strains 0384 and 0390 to serve as a positive control.