TABLE 4.

Sphingolipid levels in C. auris 0384, 0385, and 0390 strains with or without treatment

Lipid classb	Sphingolipid levels (pmol/pmol Pi) for treatment and straina:
	0384						0385						0390
	No drug		0.06 μg/ml myriocin + 4 μg/ml flucytosine		0.25 μg/ml myriocin + 2 μg/ml flucytosine		No drug		0.5 μg/ml myriocin + 2 μg/ml flucytosine		1 μg/ml myriocin + 2 μg/ml flucytosine		No drug		1 μg/ml myriocin + 4 μg/ml flucytosine		8 μg/ml myriocin + 4 μg/ml flucytosine
	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM
SPH	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.48	0.09	0.54	0.10	0.51	0.10
SPH-1-P	0.44	0.08	0.06	0.02	0.05	0.01	0.06	0.02	1.45	0.30	0.01	0.00	0.06	0.02	0.09	0.03	0.07	0.03
DHS	2.90	0.51	1.00	0.19	0.69	0.12	1.36	0.25	0.76	0.13	0.39	0.09	1.27	0.22	1.03	0.19	0.49	0.09
DHS-1-P	1.97	0.38	0.10	0.03	0.04	0.02	0.39	0.09	0.74	0.14	0.02	0.01	0.38	0.07	0.17	0.03	0.06	0.01
PHS	0.52	0.09	0.50	0.10	0.51	0.09	0.38	0.07	0.31	0.06	0.20	0.04	1.67	0.32	2.35	0.44	2.16	0.38
PHS-1-P	0.36	0.06	0.15	0.03	0.06	0.02	0.02	0.01	1.18	0.24	0.00	0.00	0.81	0.15	0.53	0.18	3.72	0.79
18:0;2/18:0;0 DHC	4.04	0.80	1.91	0.40	1.57	0.29	11.55	2.20	3.81	0.71	3.78	0.71	12.23	2.21	11.58	2.24	5.95	1.05
18:1;2/18:0;0 CER	0.20	0.04	0.27	0.05	0.23	0.04	1.85	0.34	1.21	0.23	1.60	0.30	6.80	1.28	9.15	1.81	7.33	1.29
18:2;2/18:0;1 GlcCer	7.38	1.39	6.56	1.28	5.50	1.14	9.79	2.05	11.57	2.17	10.50	1.85	4.96	0.87	4.80	0.94	4.21	0.80
19:2;2/18:0;1 GlcCer	108.96	21.00	96.11	18.04	76.36	16.48	114.09	21.37	151.82	27.48	146.29	25.72	93.52	17.18	95.84	17.87	96.07	16.86
18:0;3/24:1;1 OH-PHC	14.88	2.64	16.54	3.01	15.56	2.96	15.03	2.68	12.12	2.32	16.37	3.14	11.94	2.32	17.54	3.89	10.92	2.00
18:0;3/18:0;0 PHC	0.15	0.03	0.16	0.03	0.16	0.03	0.97	0.18	0.84	0.17	1.00	0.21	1.31	0.27	1.94	0.38	2.01	0.36
18:0;3/24:0;0 PHC	1.12	0.20	1.09	0.22	1.06	0.21	7.26	1.32	5.86	1.08	8.35	1.54	11.01	2.18	20.54	4.10	19.44	3.44
18:0;3/24:0;1 PHC	1.66	0.29	1.90	0.35	1.61	0.33	4.93	0.94	3.97	0.76	6.01	1.05	2.39	0.43	2.72	0.57	3.10	0.54
IPC 36C - 18:0;2/18:0;0	0.70	0.13	0.51	0.09	0.83	0.18	0.87	0.16	0.50	0.09	0.30	0.06	2.39	0.43	0.91	0.16	0.83	0.16
IPC 36C - 18:0;3/18:0;0	1.23	0.22	1.68	0.30	3.44	0.71	0.34	0.06	0.36	0.06	0.27	0.05	0.68	0.14	0.49	0.09	0.37	0.06
IPC 36C - 18:0;3/18:0;1	0.30	0.06	0.41	0.07	0.72	0.16	0.17	0.03	0.20	0.04	0.15	0.03	0.11	0.02	0.10	0.02	0.13	0.02
IPC 42C - 18:0;3/24:0;1	5.76	1.10	5.11	1.09	17.26	3.62	2.73	0.49	2.62	0.46	2.69	0.50	2.41	0.43	2.72	0.49	2.69	0.58
IPC 44C - 18:0;3/24:0;2	5.11	0.99	4.51	0.97	16.35	3.39	4.20	0.76	4.45	0.78	4.69	0.94	2.96	0.53	2.81	0.53	2.50	0.48
IPC 44C - 18:0;3/26:0;1	3.65	0.68	2.73	0.58	14.54	3.21	4.10	0.89	2.78	0.49	3.47	0.62	4.32	0.89	3.91	0.76	3.65	0.64
IPC 44C - 18:0;3/26:0;2	1.30	0.25	1.01	0.20	5.07	1.10	1.17	0.21	1.03	0.19	1.33	0.25	1.04	0.19	1.19	0.22	0.63	0.12

^aPi, inorganic phosphate.

^bSPH, sphingosine; SPH-1-P, sphingosine-1-phosphate; DHS, dihydrosphingosine; DHS-1-P, dihydrosphingosine-1-phosphate; PHS, phytosphingosine; PHS-1-P, phytosphingosine-1-phosphate; DHC, dihydroceramide; CER, ceramide; GlcCer, glucosylceramide; OH-PHC, hydroxylated-phytoceramide; PHC, phytoceramide; IPC, inositol phosphoryl ceramide.