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Deleting liaR delayed the emergence of DAP resistance. 503FΔliaR and 515FΔliaR were adapted to DAP resistance via flask transfer (FT) (five populations each) or bioreactor (BR) (one population each). Horizontal red line indicates clinical DAP susceptibility cutoff. Each replicate FT population was transferred to identical DAP concentrations. HOU503 data are replotted from Prater et al. (13).
