Skip to main content
. 2021 Mar 18;65(4):e01317-20. doi: 10.1128/AAC.01317-20

FIG 3.

FIG 3

Flask-transfer isolates with mutations in yvcRS repelled DAP. (a) qPCR of dltA transcripts for 503FΔliaR isolates using gdhIV as reference. All changes were statistically significant (P < 0.05, Mann-Whitney U test). (b) qPCR of dltA transcripts for 515FΔliaR isolates using gdhIV as reference. Circles, biological replicates; bars, means. (c) Isolates were incubated with 32 μg/ml BDP-DAP. Scale bars, 1 μm. E. faecalis R712 acts as a control showing the DAP redistribution phenotype. (d) Quantification of BDP:DAP. (e) Quantification of PLL:FITC; images found in Fig. S1 in the supplemental material. Isolate names in orange are 503FΔliaR isolates, and names in purple are 515FΔliaR isolates. *, Significance compared to the ancestor (P < 0.05) using Mann-Whitney U test with post hoc Holm-Bonferroni adjustment. Experiment performed in duplicate on separate days. Quantification using ImageJ.